The FLIPR assay was done on FDSS/µCell with ligand addition at 10?s with the binding assay and mAb1/isotype addition at ten?s accompanied by hCCL1 addition at three hundred?s for the Levels of competition binding assay, with a total monitoring of five hundred?s. The excitation and emission wavelengths were being set at 485?nm and 525?nm, respectiv